Buffers and pH Meter | MIT Digital Lab Techniques Manual

Making Buffers and Using a pH Meter Many chemistry and biochemistry procedures will require you to perform a reaction at a specific pH. This video demonstrates two methods of buffer preparation and proper use of the pH meter. Created by Dr. Kimberly Berkowski and Eileen Huang. View the complete resource at: ocw.mit.eduLicense: Creative Commons BY-NC-SA More information at ocw.mit.edu More courses at ocw.mit.edu WARNING NOTICE The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented. Legal Notice - ocw.mit.edu
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This course is part of a series taught by Kevin Ahern at Oregon State University on General Biochemistry. For more information about online courses go to ecampus.oregonstate.edu www.youtube.com 1. Protein structure dictates protein function. The structure of a protein is a function of the sequence of amino acids comprising it. 2. Amino acids are the monomeric (building block) units of proteins. They are covalently joined together in peptide bonds to make proteins (polypeptides). 3. There are 20 amino acids commonly found in proteins and of these 20, 19 have a chiral center and thus can exist in two stereoisomeric forms. The only one that doesn't have a chiral center is glycine. Almost all biologically made amino acids are in the same stereoisomeric form - the 'L' form. The 'D' form occurs only in very rare peptides, such as in the cell wall of bacteria. 4. Amino acids are grouped into several structural categories based on the composition of their R groups - simple, aliphatic, aromatic, sulfhydryl bond, cyclic, aliphatic hydroxyl, R-amino (your book calls these basic because they have R groups with relatively high pKa's), carboxyamides, and acidic (have R groups with relatively low pKa's). You will need to know the names of the 20 amino acids of proteins, which of the groups above each one belongs to and will need to be able to predict ionization at given pH values if you are supplied pKa values. The problem solving videos I'll be posting soon illustrate this. Of these ...