Passaging Human Pluripotent Stem Cells Using STEMium® and StemDS®

SCRIPT: In this video, we will demonstrate how to use STEMium- our proprietary serum-free, feeder-free human pluripotent stem cell growth medium and StemDS- a specially formulated stem cell dissociation solution. The following procedure was developed for passaging human pluripotent stem cells, splitting in a 1 to 3 ratio. Please keep in mind that further optimization may be needed for other cell lines. It is also important that users become thoroughly familiar with the entire method before proceeding, as culturing human pluripotent stem cells may require different laboratory techniques than other media and cell lines. List of reagents : The following reagents are required: BD Matrigel coated plate, instructions for this preparation can be found in our online video library, DPBS, complete STEMium, StemDS, pipette tips, serological pipettes, pipettors, laminar flow hood, 37 degree celsius 5% carbon dioxide incubator and an inverted microscope. Microscope zoomed up video and 2 still images of healthy cell: Prior to passaging, cells should be examined daily under an inverted microscope for overall confluency and differentiation. (image 1 and 2) Morphology of H9 human embryonic stem cells grown under feeder-free condition and in STEMium will be different compared to other media. image 3 Confluent still image Cells should be subcultured when they remain in log phase and cell colonies are nearly touching, between 70 to 75 percent confluent, as suboptimal passaging may result in ...
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The XL100 is now available with liquid handling. The XL100 allows for scanning barcodes, weighing vials, decapping screw cap closures, recapping screw cap closures, and now liquid handling. BioMicroLab's first liquid handling option for the XL100 is an electronic pipette module for dispensing volumes in the 5uL to 200uL range. The electronic pipette module is a 4th axis which works in conjunction with the existing pick and place capabilities. Look for higher volume dispensing with the XL100 later this year.